Fig. 1. Schematic diagram of the topical treatment. (A) Differences in the 2D and 3D culture methods, as well as the advantages of MSCs and 3D MSCs. The arrow represents the shaking motion applied to the culture medium within the culture flask. (B) Schedule of the cream processing and experimental procedures. IMQ was topically applied from days 0∼6, and MSC conditioned media cream applied from days 8∼10. The experiment was terminated on day 12. 2D: two-dimensional, WJ: Wharton’s jelly, CM: conditioned media, 3D: three-dimensional, MSCs: mesenchymal stem cells, IMQ: imiquimod.